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KMID : 0613820120220111587
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Journal of Life Science
2012 Volume.22 No. 11 p.1587 ~ p.1594
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3C (Chromatin Conformation Capture): A Technique to Study Chromatin Organization
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Kim Yea-Woon
Kim Ae-Ri
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Abstract
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3C (chromatin conformation capture) is a technique to analyze chromatin organization in nuclei of eukaryotic cells. The procedure of 3C includes the formaldehyde treatment of cells to fix interactions between proteins and between proteins and DNA in chromatin, the digestion of fixed chromatin with restriction enzyme, and the ligation of fragmented DNA. The efficiency of DNA ligation represents proximity between DNA fragments in chromatin organization. Studies in the β-globin locus using 3C showed that the locus control region is in close proximity to the transcriptionally-active globin genes, indicating that chromatin organization has a role in transcriptional regulation of the genes. 3C has been advanced by combining with ChIP and genome-wide sequencing. This review presents the principle and procedure of the 3C technique, the chromatin organization of the β-globin locus explained by 3C, and advanced techniques based on 3C.
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KEYWORD
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3C (chromatin conformation capture), chromatin organization, β-globin locus
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